Glutathione-Dependent Enzymes - Biomarkers of Inflammatory Process in Patients with Cellulitis of Maxillofacial Area

Introduction. Inflammation-destructive process, accompanying cellulitis, leads to the disturbances of metabolic processes and an imbalance of the antioxidant defense system. Increased free radical generation and lipid peroxidation has been considered to play an important role in the pathogenesis of cellulitis. Salivary parameters (components) reflect the patients’ metabolic state and have clinical-diagnostic significance. Aim. To examine glutathione enzymatic redox-system, including glutathione, glutathione reductase, glutathione peroxidase and glutathioneS-transferase in plasma and leucocytes of the patients with cellulitis of maxillofacial area, treated with complex therapy. Material and Methods. 20 patients (18-45 years old) and 15 healthy subjects were examined. Patients were treated with comprehensive therapy, including antioxidant remedy “Aevitum” (35 mg retinol acetate and 100 mg α-tocopherol acetate) during 7 days. The activities of antioxidant glutathione-dependent enzymes and content of glutathione were determined in blood plasma and leucocytes of the patients with cellulitis using spectrophotometry. Results. Inflammation process led to the imbalance of antioxidant glutathione-dependent defense system in patients with cellulitis. The results suggest that complex therapy with “Aevitum” was effective and partially restored imbalance of the antioxidant defense. Spearman’s correlation analysis showed positive correlation between glutathione-dependent enzymes in blood plasma and leucocytes of the patients with cellulitis in a week time of complex therapeutic course. Conclusion. Glutathione-dependent enzymes may be biomarkers of the inflammation process in patients with cellulitis; their activities reflect a degree of pathological process activity.